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Anti-Cyclin D3/CCND3 Antibody (A92900)

Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900) - Antibodies.com Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to Cyclin D3/CCND3 for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cyclin D3/CCND3 Antibody
Description
Rabbit polyclonal antibody to Cyclin D3/CCND3.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1).
Sequence
SMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin D3/CCND3 Antibody (A92900) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin D3/CCND3 Antibody (A92900)

Western blot analysis of extracts from normal (control) and Cyclin D3 knockout (KO) HeLa cells, using Anti-Cyclin D3/CCND3 Antibody (A92900) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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