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Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752)

Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.com Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.com Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.com Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.com Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Cytochrome b245 Light Chain/p22-phox for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cytochrome b245 Light Chain/p22-phox Antibody
Description
Rabbit polyclonal antibody to Cytochrome b245 Light Chain/p22-phox.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 126-195 of human CYBA (NP_000092.2).
Sequence
VRGEQWTPIEPKPRERPQIGGTIKQPPSNPPPRPPAEARKKPSEEEAAVAAGGPPGGPQVNPIPVTDEVV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
22 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.comEnlarge Image

Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752)

Western blot analysis of RAW264. 7, using Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.comEnlarge Image

Western Blot - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752)

Western blot analysis of Rat lung, using Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752)

Immunofluorescence analysis of MCF7 cells using Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Cytochrome b245 Light Chain/p22-phox Antibody (A8752) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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