Anti-Cytokeratin 1 Antibody [ARC1745] (A306008)

Name: Anti-Cytokeratin 1 Antibody [ARC1745]
Brand: Antibodies.com
SKU: A306008
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1745] antibody to Cytokeratin 1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Epithelial Cell Marker Keratinocyte Marker

Specifications

Name

Anti-Cytokeratin 1 Antibody [ARC1745]

Description

Rabbit monoclonal [ARC1745] antibody to Cytokeratin 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (KRT1) (P04264).

Sequence

GGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1745

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

67 kDa cytokeratin, CK-1, Cytokeratin-1, Hair alpha protein, K1, Keratin, type II cytoskeletal 1, Keratin-1, KRT1, KRTA, Type-II keratin Kb1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-Cytokeratin 1 Antibody [ARC1745] (A306008)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 1 Antibody [ARC1745] (A306008) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-Cytokeratin 1 Antibody [ARC1745] (A306008)

Western blot analysis of extracts of A375 cells, using Anti-Cytokeratin 1 Antibody [ARC1745] (A306008) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-Cytokeratin 1 Antibody [ARC1745] (A306008)

Immunohistochemistry analysis of paraffin-embedded rat skin using Anti-Cytokeratin 1 Antibody [ARC1745] (A306008) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Cytokeratin 1 Antibody [ARC1745] (A306008)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 1 Antibody [ARC1745] (A306008) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.