Mouse monoclonal [DE-K13] antibody to Cytokeratin 10 + 13.
Specificity
The antibody recognizes only Cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.
Applications
WB, IHC-P, IHC-Fr
Dilutions
IHC-P: Standard ABC technique (DAB+), recommended dilution: 10 µg/ml (1:100), Positive Control: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13, IHC-Fr: Use at an assay dependent dilution, WB: 1-3 µg/ml.
Reactivity
Feline, Human
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium.
Host
Mouse
Clonality
Monoclonal
Clone ID
DE-K13
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CK-10, CK10, CK13, Cytokeratin-10, K10, K13, K1C10_HUMAN, Keratin, Keratin 10, Keratin 13, Keratin-10, KRT10, KRT13, type I cytoskeletal 10
Immunohistochemistry staining of tonsil (paraffin-embedded sections) with Anti-Cytokeratin 10 + 13 Antibody (A85818). Anti-Cytokeratin 10 + 13 Antibody (A85818) stains only Cytokeratin 13 when used on formalin-fixed, paraffin-embedded tissue sections.
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