Anti-Cytokeratin 4 Antibody [ARC1804] (A307427)

Name: Anti-Cytokeratin 4 Antibody [ARC1804]
Brand: Antibodies.com
SKU: A307427
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1804] antibody to Cytokeratin 4 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cytokeratin 4 Antibody [ARC1804]

Description

Rabbit monoclonal [ARC1804] antibody to Cytokeratin 4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 4 (KRT4) (P19013).

Sequence

MIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVAGSRQGACFGGAGGFGTGGFGGGFGGSFSGKGGPG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1804

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CK-4, CYK4, Cytokeratin-4, K4, Keratin, type II cytoskeletal 4, Keratin-4, KRT4, Type-II keratin Kb4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cytokeratin 4 Antibody [ARC1804] (A307427)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 4 Antibody [ARC1804] (A307427) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Cytokeratin 4 Antibody [ARC1804] (A307427)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Anti-Cytokeratin 4 Antibody [ARC1804] (A307427) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Cytokeratin 4 Antibody [ARC1804] (A307427)

Immunofluorescence analysis of rat rectum using Anti-Cytokeratin 4 Antibody [ARC1804] (A307427) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Cytokeratin 4 Antibody [ARC1804] (A307427)

Immunofluorescence analysis of mouse large intestine using Anti-Cytokeratin 4 Antibody [ARC1804] (A307427) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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