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Recombinant Anti-Cytokeratin 8 + 18 Antibody [KRT8.18/6579R] (A278106)

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Recombinant Antibody
$580
Product Datasheet

Recombinant rabbit monoclonal [KRT8.18/6579R] antibody to Cytokeratin 8 + 18 for Flow Cytometry, IF, WB and IHC-P.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Cytokeratin 8 + 18 Antibody [KRT8.18/6579R]
Description
Recombinant rabbit monoclonal [KRT8.18/6579R] antibody to Cytokeratin 8 + 18.
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human Cytokeratin 8 proteins.
Host
Rabbit
Clonality
Oligoclonal
Clone ID
KRT8.18/6579R
Isotype
IgG, kappa + IgG, kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
52.5 kDa (CK8) / 45 kDa (CK18)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes

Please note, this product is an oligoclonal antibody; it is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies not only have the specificity and batch-to-batch consistency of a monoclonal antibody, but they also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

This oligoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Cytokeratin 8 + 18 Antibody [KRT8.18/6579R] - BSA and Azide free (A278694).

Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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