This antibody recognises cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. recognises the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting.
Applications
WB, ELISA
Dilutions
ELISA: 1:500 - 1:2500, WB: 1:100 - 1:500
Reactivity
S. cerevisiae
Immunogen
Purified yeast cytosine deaminase.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein G affinity chromatography.
Concentration
5 mg/ml
Molecular Weight
Approximately 13 kDa and 17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti cytosine deaminase antibody recognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004).
