Anti-DBT Antibody (A308791)

Name: Anti-DBT Antibody
Brand: Antibodies.com
SKU: A308791
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to DBT for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-DBT Antibody

Description

Rabbit polyclonal antibody to DBT.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3).

Sequence

SRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

2-methylpropanoyl)transferase, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, BCATE2, BCKAD-E2, BCKADE2, BCKDH-E2, BCKDHE2, BCOADC-E2, Branched chain 2-oxo-acid dehydrogenase complex component E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue, Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-DBT Antibody (A308791)

Western blot analysis of extracts of various cell lines, using Anti-DBT Antibody (A308791) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-DBT Antibody (A308791)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-DBT Antibody (A308791) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-DBT Antibody (A308791)

Immunofluorescence analysis of A-431 cells using Anti-DBT Antibody (A308791) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-DBT Antibody (A308791)

Immunofluorescence analysis of PC-12 cells using Anti-DBT Antibody (A308791) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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