Mouse monoclonal [F-tag-01] antibody to DDDDK Tag.
Specificity
The antibody recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Applications
ICC, WB
Reactivity
Species Independent
Immunogen
Synthetic peptide: DYKDDDDK conjugated to KLH.
Sequence
DYKDDDDK
Host
Mouse
Clonality
Monoclonal
Clone ID
F-tag-01
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained using Anti-DDDDK Tag Antibody [F-tag-01] (A85906), (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)B - cell nuclei stained with DAPI (blue)C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)
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