Western blot analysis of extracts of various cell lines, using Anti-DDX3 Antibody (A14874) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-DDX3 Antibody (A14874) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-DDX3 Antibody (A14874) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-DDX3 Antibody (A14874) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 600µg extracts of mouse brain using 3µg of Anti-DDX3 Antibody (A14874). This Western blot was performed on the immunoprecipitate using Anti-DDX3 Antibody (A14874) at a dilution of 1:1000.
Immunoprecipitation analysis of 300µg extracts of C6 cells using 3µg of Anti-DDX3 Antibody (A14874). This Western blot was performed on the immunoprecipitate using Anti-DDX3 Antibody (A14874) at a dilution of 1:3000.
