Mouse monoclonal [15E2] antibody to Dectin-1 (PE).
Specificity
This antibody recognizes an extracellular epitope, LWEDGSTFSSN, of human CD369, a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Extracellular part of human CD369 with hIgG FC tag.
Host
Mouse
Clonality
Monoclonal
Clone ID
15E2
Isotype
IgG2a
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
33 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Beta-glucan receptor, BGR, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CLEC7A Antibody [15E2] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
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