Anti-DEK Antibody [ARC1825] (A308723)

Name: Anti-DEK Antibody [ARC1825]
Brand: Antibodies.com
SKU: A308723
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1825] antibody to DEK for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-DEK Antibody [ARC1825]

Description

Rabbit monoclonal [ARC1825] antibody to DEK.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 51-200 of human DEK (P35659).

Sequence

SLIVEGKREKKKVERLTMQVSSLQREPFTIAQGKGQKLCEIERIHFFLSKKKTDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSVQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKTC

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1825

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein DEK

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-DEK Antibody [ARC1825] (A308723)

Western blot analysis of extracts of various cell lines, using Anti-DEK Antibody [ARC1825] (A308723) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunohistochemistry - Anti-DEK Antibody [ARC1825] (A308723)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-DEK Antibody [ARC1825] (A308723) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-DEK Antibody [ARC1825] (A308723)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-DEK Antibody [ARC1825] (A308723) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-DEK Antibody [ARC1825] (A308723)

Immunofluorescence analysis of C6 cells using Anti-DEK Antibody [ARC1825] (A308723) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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