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Anti-DENDRA2 Antibody (A121799)

WB - Anti-DENDRA2 Antibody (A121799) Flow Cytometry - Anti-DENDRA2 Antibody (A121799) Flow Cytometry - Anti-DENDRA2 Antibody (A121799)
$210
Product Datasheet

Rabbit polyclonal antibody to DENDRA2 for WB, ICC and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-DENDRA2 Antibody
Description
Rabbit polyclonal antibody to DENDRA2.
Specificity
This antibody recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya).
Applications
WB, ICC, Flow Cytometry
Dilutions
WB: 1-2 µg/ml, Flow Cytometry: 1-4 µg/ml; Extracellular and Intracellular staining.
Reactivity
Species Independent
Immunogen
DENDRA2 tag.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

WB - Anti-DENDRA2 Antibody (A121799)Enlarge Image

Anti-DENDRA2 Antibody (A121799)

Western blotting analysis of Dendra2/GPI fusion protein using Anti-DENDRA2 Antibody (A121799) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct; reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of anti-Dendra2 rabbit polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for Dendra2/GPI fusion protein at approximately 35 kDa.
Flow Cytometry - Anti-DENDRA2 Antibody (A121799)Enlarge Image

Flow Cytometry - Anti-DENDRA2 Antibody (A121799)

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct labeling DENDRA2 with Anti-DENDRA2 Antibody (A121799), (concentration in sample 2 µg/ml, GAR APC).
Flow Cytometry - Anti-DENDRA2 Antibody (A121799)Enlarge Image

Flow Cytometry - Anti-DENDRA2 Antibody (A121799)

Separation of HEK293T/17 cells transfected using Anti-DENDRA2 Antibody (A121799), (concentration in sample 2 µg/ml, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.
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