Synthetic peptide within amino acids 1200 and the C-terminus of human DIAPH1 (NP_005210. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
DIAP1, Diaphanous-related formin-1, DRF1, Protein diaphanous homolog 1
Samples: Whole cell lysate (50 µg/lane) from untreated or siRNA treated HEK293T cells. Antibody: DIAPH1 Antibody (A295241) was used at 0.1 µg/ml. Detection: Chemiluminescence with 10 second exposure.
Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: DIAPH1 Antibody (A295241) was used for WB at 0.66 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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