Anti-DMT1 Antibody [ARC59972] (A309631)

Name: Anti-DMT1 Antibody [ARC59972]
Brand: Antibodies.com
SKU: A309631

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This product is discontinued

Anti-DMT1 Antibody [ARC59972] (A309631) has been discontinued and is no longer available.

View all DMT1 Antibodies.

Specifications

Name

Anti-DMT1 Antibody [ARC59972]

Description

Rabbit monoclonal [ARC59972] antibody to DMT1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant protein of human DMT1/SLC11A2.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59972

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 - 100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT-1, Natural resistance-associated macrophage protein 2, NRAMP 2, NRAMP2, SLC11A2, Solute carrier family 11 member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-DMT1 Antibody [ARC59972] (A309631)

Western blot analysis of 293F, using Anti-DMT1 Antibody [ARC59972] (A309631) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunofluorescence - Anti-DMT1 Antibody [ARC59972] (A309631)

Immunofluorescence analysis of 293T cells using Anti-DMT1 Antibody [ARC59972] (A309631) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-DMT1 Antibody [ARC59972] (A309631)

Immunoprecipitation analysis of 300µg extracts of 293F cells using 3µg of Anti-DMT1 Antibody [ARC59972] (A309631). This Western blot was performed on the immunoprecipitate using Anti-DMT1 Antibody [ARC59972] (A309631) at a dilition of 1:1000.