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Anti-DNA Ligase IV/LIG4 Antibody [ARC0595] (A80811)

Western Blot - Anti-DNA Ligase IV/LIG4 Antibody (A80811) - Antibodies.com
$515
Product Datasheet

Rabbit monoclonal [ARC0595] antibody to DNA Ligase IV/LIG4 for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-DNA Ligase IV/LIG4 Antibody [ARC0595]
Description
Rabbit monoclonal [ARC0595] antibody to DNA Ligase IV/LIG4.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917).
Sequence
SRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEF
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0595
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-DNA Ligase IV/LIG4 Antibody (A80811) - Antibodies.comEnlarge Image

Western Blot - Anti-DNA Ligase IV/LIG4 Antibody [ARC0595] (A80811)

Western blot analysis of extracts of various cell lines, using Anti-DNA Ligase IV/LIG4 Antibody (A80811) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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