Anti-DNA Polymerase beta Antibody [ARC0752] (A307402)

Name: Anti-DNA Polymerase beta Antibody [ARC0752]
Brand: Antibodies.com
SKU: A307402
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0752] antibody to DNA Polymerase beta for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-DNA Polymerase beta Antibody [ARC0752]

Description

Rabbit monoclonal [ARC0752] antibody to DNA Polymerase beta.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 236-335 of human DNA Polymerase beta (P06746).

Sequence

MGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0752

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, POLB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-DNA Polymerase beta Antibody [ARC0752] (A307402)

Western blot analysis of extracts of various cell lines, using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-DNA Polymerase beta Antibody [ARC0752] (A307402)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-DNA Polymerase beta Antibody [ARC0752] (A307402)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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