Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, DNAJ2, hDj-2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ-2, HSJ2, HSPF4, Human DnaJ protein 2
Western blot analysis of extracts from wild type(WT) and DNAJA1 knockout (KO) 293T(KO) cells, using Anti-DNAJA1 Antibody (A80686) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-DNAJA1 Antibody (A80686) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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