Anti-Dnmt3b Antibody (A11580)

Name: Anti-Dnmt3b Antibody
Brand: Antibodies.com
SKU: A11580
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Dnmt3b for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Dnmt3b Antibody

Description

Rabbit polyclonal antibody to Dnmt3b.

Applications

WB, ICC/IF

Dilutions

WB: 1:200-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-475 of human DNMT3B (Q9UBC3).

Sequence

IEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Dnmt3b Antibody (A11580)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt3b Antibody (A11580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-Dnmt3b Antibody (A11580)

Immunofluorescence analysis of C6 cells using Anti-Dnmt3b Antibody (A11580) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Dnmt3b Antibody (A11580)

Immunofluorescence analysis of HeLa cells using Anti-Dnmt3b Antibody (A11580) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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