Anti-DNP Antibody (CF®640R) (A344027)

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

Relative photostability of CF®640R and Alexa Fluor® 647 fluorescence. HeLa cells were stained with mouse anti-tubulin primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter set, and images were acquired at time zero and after 1 and 3 minutes of light exposure.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.

CF®640R produces brighter antibody conjugates than other far-red dyes. Jurkat cells were stained with mouse anti-CD3 primary antibody or without primary antibody as a control, followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Fluorescence was analyzed on a BD FACSCalibur™ flow cytometer using the FL4 detection channel, and bars represent geometric mean fluorescence intensity.

CF®640R exhibits substantially greater photostability than Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter cube, with images acquired every 15 seconds for 5 minutes. Fluorescence intensity was normalized to the initial time point.