Mouse monoclonal [E6-71A1] antibody to Dog IgE (Biotin).
Specificity
This antibody recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.
Applications
WB, ELISA
Reactivity
Canine
Immunogen
Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
Host
Mouse
Clonality
Monoclonal
Clone ID
E6-71A1
Isotype
IgG1
Conjugate
Biotin
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 62-65 kDa in samples of affinity purified dog IgE under reducing conditions.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.