Anti-Dopamine Transporter Antibody (A91403)

Name: Anti-Dopamine Transporter Antibody
Brand: Antibodies.com
SKU: A91403
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Dopamine Transporter for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Dopaminergic Neuron Marker

Specifications

Name

Anti-Dopamine Transporter Antibody

Description

Rabbit polyclonal antibody to Dopamine Transporter.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human Dopamine Transporter/SLC6A3 (NP_001035.1).

Sequence

GVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DA transporter, DAT, DAT1, SLC6A3, Sodium-dependent dopamine transporter, Solute carrier family 6 member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Dopamine Transporter Antibody (A91403)

Western blot analysis of Rat brain, using Anti-Dopamine Transporter Antibody (A91403) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Dopamine Transporter Antibody (A91403)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Dopamine Transporter Antibody (A91403)

Immunofluorescence analysis of rat brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Dopamine Transporter Antibody (A91403)

Immunofluorescence analysis of mouse brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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