Anti-DOT1L Antibody (A12763)

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This product is discontinued

Anti-DOT1L Antibody (A12763) has been discontinued and is no longer available.

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Specifications

Name

Anti-DOT1L Antibody

Description

Rabbit polyclonal antibody to DOT1L.

Applications

WB, IHC, IF

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human DOT1L (NP_115871.1).

Sequence

TDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLSGPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

170kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Histone-lysine N-methyltransferase, H3 lysine-79 specific, KIAA1814, KMT4, Lysine N-methyltransferase 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-DOT1L Antibody (A12763)

Western blot analysis of extracts of various cell lines, using MRPL39 antibody (A10023) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.