This antibody binds specifically to the C-terminal domain of human DRD1, detecting the non-phosphorylated form. It is optimized for Western blot analysis, supports immunoprecipitation from cell and tissue lysates, and is suitable for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human DRD1. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical detection of Dopamine Receptor 1 (DRD1) in striatum. Striatal sections were dewaxed, microwaved in citric acid, and stained with anti-DRD1 antibody (A334475) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Localization of Dopamine Receptor 1 (DRD1) and Dopamine Receptor 2 (DRD2) in striatum by immunohistochemistry. Striatal sections were dewaxed, microwaved in citric acid, and incubated with either anti-DRD1 antibody (A334475) at 1:100 (left panel) or anti-DRD2 antibody (A334504) at 1:100 (right panel), followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Immunocytochemical detection of Dopamine Receptor 1 (DRD1) in transfected HEK293 cells. HEK293 cells stably expressing DRD1 were untreated or treated with 100 nM Dopamine for 30 min and stained with anti-DRD1 antibody (A334475) at 1:200. DRD1 receptors were localized to the plasma membrane in untreated cells (MOCK) and in perinuclear vesicular clusters after 30 min of Dopamine exposure.
Western blot validation of Dopamine Receptor 1 (DRD1) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing DRD1 were probed with the phosphorylation-independent anti-DRD1 antibody (A334475) at a 1:1000 dilution.
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