Anti-DRD2 Antibody (A334505)

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This product is discontinued

Anti-DRD2 Antibody (A334505) has been discontinued and is no longer available.

View all DRD2 Antibodies.

Specifications

Name

Anti-DRD2 Antibody

Description

Rabbit polyclonal antibody to DRD2.

Specificity

This antibody binds specifically to the third intracellular loop of human DRD2, detecting the non-phosphorylated isoform. It is validated for Western blot analysis, enables immunoprecipitation from brain tissue lysates, and supports immunohistochemistry in cultured cells and tissue sections.

Applications

WB, ICC, IHC

Dilutions

WB: 1,000, ICC: 1:200

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to C-terminal of human DRD2. Immunogen range is 22-22 amino acids.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

D(2) dopamine receptor, Dopamine D2 receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Anti-DRD2 Antibody (A334505)

Western blot confirmation of Dopamine Receptor 2 (DRD2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing DRD2 were immunoblotted with the phosphorylation-independent anti-DRD2 antibody (A334505) at a 1:1000 dilution.

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Immunohistochemistry - Anti-DRD2 Antibody (A334505)

Detection of Dopamine Receptor 1 (DRD1) and Dopamine Receptor 2 (DRD2) in striatum by immunohistochemistry. Striatal sections were dewaxed, microwaved in citric acid, and stained with either anti-DRD1 antibody (A334475) at 1:100 (left panel) or anti-DRD2 antibody (A334504) at 1:100 (right panel), followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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Immunohistochemistry - Anti-DRD2 Antibody (A334505)

Immunohistochemical localization of Dopamine Receptor 2 (DRD2) in hypothalamus. Hypothalamic sections were dewaxed, microwaved in citric acid, and stained with anti-DRD2 antibody (A334505-I) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. DRD2 receptors were detected in a distinct population of neuroendocrine tumor cells.

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Immunohistochemistry - Anti-DRD2 Antibody (A334505)

Detection of Dopamine Receptor 2 (DRD2) in human small cell lung carcinoma by immunohistochemistry. Tumor sections were dewaxed, microwaved in citric acid, and incubated with anti-DRD2 antibody (A334505) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. DRD2 receptors were observed in a specific subset of carcinoma cells.