Sheep polyclonal antibody to Dynamin (phospho Ser774).
Specificity
This antibody recognises dynamin, also known as B-dynamin and D100, when phosphorylated at serine 774.Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Applications
WB, IHC-Fr, IF
Dilutions
WB: 1:1,000
Reactivity
Rat, Human, Monkey, Bovine, Chicken, Mouse, Dog
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser774.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Molecular Weight
Approximately 95 kDa in rat hippocampus lysates.
Product Form
Liquid
Formulation
Supplied in 10mM HEPES, pH 7.5, with 0.01% BSA, 50% Glycerol, and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Rat dynamin (pSer774) antibody recognizes dynamin, also known as B-dynamin and D100, when phosphorylated at serine 774.Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
