Anti-Dysferlin Antibody [ARC2178] (A305862)

Name: Anti-Dysferlin Antibody [ARC2178]
Brand: Antibodies.com
SKU: A305862
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC2178] antibody to Dysferlin for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Dysferlin Antibody [ARC2178]

Description

Rabbit monoclonal [ARC2178] antibody to Dysferlin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (Romeo) (O75923).

Sequence

FEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2178

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

280 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DYSF, Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FER1L1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Dysferlin Antibody [ARC2178] (A305862)

Western blot analysis of extracts of RD cells, using Anti-Dysferlin Antibody [ARC2178] (A305862) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Dysferlin Antibody [ARC2178] (A305862)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Dysferlin Antibody [ARC2178] (A305862) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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