Anti-E-Cadherin Antibody [4A2] (CF®405S) (A353246)

Anti-E-Cadherin Antibody [4A2] (CF®405S)

Mouse monoclonal [4A2] antibody to E-Cadherin (CF®405S).

Flow Cytometry, IF, IHC-P, IP, WB

LS174T, Raji, HT29 and SK-BR3 cells or prostate and colon carcinomas.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human, Mouse, Rat

Recombinant full-length human E-cadherin protein.

Mouse

Monoclonal

IgG1

kappa

0.1 mg/mL

120-80 kDa (Mature); 135 kDa (Precursor)

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Cadherin-1, CAM 120/80, CD324, CDH1, CDHE, Epithelial cadherin, UVO, Uvomorulin

This product is for research use only. It is not intended for diagnostic or therapeutic use.