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Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829)

Western Blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.com Immunohistochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.com Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1493] antibody to E1 Ubiquitin Activating Enzyme 1/UBA1 for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493]
Description
Rabbit monoclonal [ARC1493] antibody to E1 Ubiquitin Activating Enzyme 1/UBA1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 959-1058 of human UBA1 (P22314).
Sequence
EVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1493
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
110 - 118 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.comEnlarge Image

Western Blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829)

Western blot analysis of extracts of various cell lines, using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829)

Immunofluorescence analysis of U-2 OS cells using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody [ARC1493] (A305829) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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