Anti-DSP Antibody (A15091)

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This product is discontinued

Anti-DSP Antibody (A15091) has been discontinued and is no longer available.

View all DSP Antibodies.

Specifications

Name

Anti-DSP Antibody

Description

Rabbit polyclonal antibody to DSP.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2750-2850 of human DSP (NP_004406.2).

Sequence

AIRKGFIDGRAAQRLQDTSSYAKILTCPKTKLKISYKDAINRSMVEDITGLRLLEAASVSSKGLPSPYNMSSAPGSRSGSRSGSRSGSRSGSRSGSRRGSF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

315kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

250/210 kDa paraneoplastic pemphigus antigen, DP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-DSP Antibody (A15091)

Western blot analysis of extracts of various cell lines, using E2F6 antibody (A6151) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Immunohistochemistry - Anti-DSP Antibody (A15091)

Immunohistochemistry of paraffin-embedded mouse lung using E2F6 antibody (A6151) at dilution of 1:200 (40x lens).

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Immunofluorescence - Anti-DSP Antibody (A15091)

Immunofluorescence analysis of U2OS cells using E2F6 antibody (A6151). Blue: DAPI for nuclear staining.

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Immunoprecipitation - Anti-DSP Antibody (A15091)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg E2F6 antibody (A6151). Western blot was performed from the immunoprecipitate using E2F6 antibody (A6151) at a dilition of 1:500.