Anti-EBI2 Antibody (A334533)

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This product is discontinued

Anti-EBI2 Antibody (A334533) has been discontinued and is no longer available.

View all EBI2 Antibodies.

Specifications

Name

Anti-EBI2 Antibody

Description

Rabbit polyclonal antibody to EBI2.

Specificity

This antibody specifically recognizes the C-terminal domain of human EBI2, detecting the non-phosphorylated isoform. It is suitable for Western blot analysis, facilitates immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.

Applications

WB, IHC

Dilutions

WB: 1,000, IHC: 1:100

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C-terminal of human EBI2. Immunogen range is 22-22 amino acids.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2, G-protein coupled receptor 183, GPR183, hEBI2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Anti-EBI2 Antibody (A334533)

Western blot analysis of EBI2 Receptor (EBI2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing EBI2, untreated or treated with 100 nM 7alpha,25-OHC for 5 min, were immunoblotted with the phosphorylation-independent anti-EBI2 antibody (A334533) at a 1:1000 dilution.

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Immunohistochemistry - Anti-EBI2 Antibody (A334533)

Immunohistochemical detection of EBI2 Receptor (EBI2) in human spleen. Spleen sections were dewaxed, microwaved in citric acid, and incubated with anti-EBI2 antibody (A334533) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. EBI2 receptors were uniformly detected at the plasma membrane of nearly all spleen cells.

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Immunohistochemistry - Anti-EBI2 Antibody (A334533)

Verification of EBI2 Receptor (EBI2) antibody specificity in human spleen by immunohistochemistry. Spleen sections were dewaxed, microwaved in citric acid, and incubated without (left panel) or with (right panel) the peptide used to generate anti-EBI2 antibody (A334533). Sections were stained with anti-EBI2 antibody (A334533) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution, then developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. EBI2 receptors were detected at the plasma membrane only in sections without peptide incubation.