This antibody specifically recognizes the C-terminal domain of human EBI2, detecting the non-phosphorylated isoform. It is suitable for Western blot analysis, facilitates immunoprecipitation from tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human EBI2. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of EBI2 Receptor (EBI2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing EBI2, untreated or treated with 100 nM 7alpha,25-OHC for 5 min, were immunoblotted with the phosphorylation-independent anti-EBI2 antibody (A334533) at a 1:1000 dilution.
Immunohistochemical detection of EBI2 Receptor (EBI2) in human spleen. Spleen sections were dewaxed, microwaved in citric acid, and incubated with anti-EBI2 antibody (A334533) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. EBI2 receptors were uniformly detected at the plasma membrane of nearly all spleen cells.
Verification of EBI2 Receptor (EBI2) antibody specificity in human spleen by immunohistochemistry. Spleen sections were dewaxed, microwaved in citric acid, and incubated without (left panel) or with (right panel) the peptide used to generate anti-EBI2 antibody (A334533). Sections were stained with anti-EBI2 antibody (A334533) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution, then developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. EBI2 receptors were detected at the plasma membrane only in sections without peptide incubation.
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Proteins predicted to interact with EBI2
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
