Western blot analysis of extracts of various cell lines, using Anti-EED Antibody (A14710) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of A-549 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-EED Antibody (A14710). This Western blot was performed on the immunoprecipitate using Anti-EED Antibody (A14710) at a dilution of 1:1000.
