Anti-EEF2K (phospho Ser366) Antibody (A87764)

Western blot analysis of extracts of HeLa cells, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution. NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of HeLa cells, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution or EEF2K antibody (A5404). Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 15s.