Mouse monoclonal [B1D8] antibody to EGFR (CF®568).
Applications
Flow Cytometry (Cell Surface), Functional studies, IP
Positive Control
A431 cells and breast or bladder carcinoma tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IP: 1-2 µg/500 µg protein lysate. For affinity purification and antibody coupling applications, use a BSA-free antibody formulation. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Cross Reactivity
No cross-reactivity with Mouse or Rat. Cross-reactivity with other species has not been determined.
Immunogen
Microsomes from A431 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
B1D8
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®568
Concentration
0.1 mg/mL
Predicted MW
~170 kDa (wild type) and ~145 kDa (vIII variant)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.