Anti-EGFR Antibody [GFR/1667] (CF®405S) (A353464)

Anti-EGFR Antibody [GFR/1667] (CF®405S)

Mouse monoclonal [GFR/1667] antibody to EGFR (CF®405S).

IHC, IHC-P, WB

A431 cells or placenta, breast, colon or Bladder cancer tissue.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 1-2 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human

Recombinant full-length human EGFR protein.

Mouse

Monoclonal

IgG1

0.1 mg/mL

~145 kDa (vIII variant), ~170 kDa (wild type)

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

This product is for research use only. It is not intended for diagnostic or therapeutic use.