Mouse monoclonal [GFR1195] antibody to EGFR (CF®488A).
Applications
IHC, IHC-P
Positive Control
A431 cells or placenta, breast, colon and bladder cancer tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 2-4 µg/mL. For IHC-P, protease-induced epitope retrieval is recommended using Protease at 1 mg/mL in PBS, pH 7.4, for 10 minutes at 37°C.
Reactivity
Human
Immunogen
Recombinant human EGFR protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
GFR1195
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®488A
Concentration
0.1 mg/mL
Predicted MW
~170 kDa (wild type) and ~145 kDa (vIII variant)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.