Anti-EGFR Antibody [GFR450] (Biotin) (A352523)

Anti-EGFR Antibody [GFR450] (Biotin)

Mouse monoclonal [GFR450] antibody to EGFR (Biotin).

Flow Cytometry, IF

A431 cells or breast and bladder carcinoma tissue.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 2-4 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes.

Human

No cross-reactivity with Mouse. Cross-reactivity with other species has not been determined.

Recombinant extracellular domain of human EGFR protein.

Mouse

Monoclonal

GFR450

kappa

0.1 mg/mL

~170 kDa (wild type) and ~145 kDa (vIII variant)

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

This product is for research use only. It is not intended for diagnostic or therapeutic use.