Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
AT225, Early growth response protein 1, EGR-1, KROX24, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, ZNF225
Western blot analysis of extracts of various cell lines, using Anti-Egr1 Antibody (A14152) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using Anti-Egr1 Antibody (A14152) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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