This antibody recognizes human EIF 2 alpha only when phosphorylated at Ser51.
Applications
WB, IHC
Dilutions
IHC: 1:100-1:200, WB: 1:100-1:206
Reactivity
Human
Cross Reactivity
This antibody does not cross-react with EIF 2 alpha without phosphorylation at Ser51. This antibody may react with samples from mouse and rat, as predicted by immunogen homology.
Immunogen
Synthetic peptide corresponding to human EIF 2 alpha (phospho Ser51).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM298
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] at a 1:200 dilution.
Western Blot - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245)
Western blot of HeLa cell lysate non-treated (-) or treated (+) with Calyculin A (CalA), using Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] at a 1:200 dilution.
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![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121479) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_1.png?profile=product_top)
![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_2.png?profile=product_top)
![Western Blot - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_3.png?profile=product_top)
![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121479) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_1.png?profile=product_top_thumb)
![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_2.png?profile=product_top_thumb)
![Western Blot - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_3.png?profile=product_top_thumb)
![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121479) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_1.png?profile=product_image)
![Immunohistochemistry - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_2.png?profile=product_image)
![Western Blot - Anti-EIF 2 alpha (phospho Ser51) Antibody [RM298] (A121245) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121245_3.png?profile=product_image)