Anti-eIF2A Antibody [ARC1712] (A305361)

Name: Anti-eIF2A Antibody [ARC1712]
Brand: Antibodies.com
SKU: A305361
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1712] antibody to eIF2A for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-eIF2A Antibody [ARC1712]

Description

Rabbit monoclonal [ARC1712] antibody to eIF2A.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44).

Sequence

ALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1712

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

65 kDa eukaryotic translation initiation factor 2A, eIF-2A, Eukaryotic translation initiation factor 2A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-eIF2A Antibody [ARC1712] (A305361)

Western blot analysis of extracts of various cell lines, using Anti-eIF2A Antibody [ARC1712] (A305361) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-eIF2A Antibody [ARC1712] (A305361)

Western blot analysis of extracts of Rat lung, using Anti-eIF2A Antibody [ARC1712] (A305361) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-eIF2A Antibody [ARC1712] (A305361)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using Anti-eIF2A Antibody [ARC1712] (A305361) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-eIF2A Antibody [ARC1712] (A305361)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-eIF2A Antibody [ARC1712] (A305361) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.