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Anti-EIF3S1/EIF3J Antibody (A89528)

Western Blot - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.com Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.com Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.com Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to EIF3S1/EIF3J for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-EIF3S1/EIF3J Antibody
Description
Rabbit polyclonal antibody to EIF3S1/EIF3J.
Applications
WB, ICC/IF
Dilutions
WB: 1:200-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
Sequence
MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.comEnlarge Image

Western Blot - Anti-EIF3S1/EIF3J Antibody (A89528)

Western blot analysis of extracts of various cell lines, using Anti-EIF3S1/EIF3J Antibody (A89528) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528)

Immunofluorescence analysis of HeLa cells using Anti-EIF3S1/EIF3J Antibody (A89528) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528)

Immunofluorescence analysis of NIH/3T3 cells using Anti-EIF3S1/EIF3J Antibody (A89528) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-EIF3S1/EIF3J Antibody (A89528)

Immunofluorescence analysis of PC-12 cells using Anti-EIF3S1/EIF3J Antibody (A89528) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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