Western blot analysis of extracts of various cell lines, using Anti-EIF4B Antibody (A5405) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Immunoprecipitation analysis of 200µg extracts of 293T cells using 1µg Anti-EIF4B Antibody (A5405). Western blot was performed from the immunoprecipitate using Anti-EIF4B Antibody (A5405) at a dilition of 1:1000.