Anti-EIF4E2 Antibody (A14549)

Name: Anti-EIF4E2 Antibody
Brand: Antibodies.com
SKU: A14549

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This product is discontinued

Anti-EIF4E2 Antibody (A14549) has been discontinued and is no longer available.

View all EIF4E2 Antibodies.

Specifications

Name

Anti-EIF4E2 Antibody

Description

Rabbit polyclonal antibody to EIF4E2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF4E2 (NP_004837.1).

Sequence

MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

27 kDa / 28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

eIF-4E type 2, eIF4E-like protein 4E-LP, EIF4EL3, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E type 2, Eukaryotic translation initiation factor 4E-like 3, h4EHP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-EIF4E2 Antibody (A14549)

Western blot analysis of extracts of various cell lines, using Anti-EIF4E2 Antibody (A14549) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 2s.

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Western Blot - Anti-EIF4E2 Antibody (A14549)

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Immunofluorescence - Anti-EIF4E2 Antibody (A14549)

Immunofluorescence analysis of C6 cells using Anti-EIF4E2 Antibody (A14549) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-EIF4E2 Antibody (A14549)

Immunofluorescence analysis of NIH-3T3 cells using Anti-EIF4E2 Antibody (A14549) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).