Anti-Elongation factor 1-gamma Antibody (A90247)

Name: Anti-Elongation factor 1-gamma Antibody
Brand: Antibodies.com
SKU: A90247
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Elongation factor 1-gamma for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Elongation factor 1-gamma Antibody

Description

Rabbit polyclonal antibody to Elongation factor 1-gamma.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human eEF1G (NP_001395.1).

Sequence

LLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

eEF-1B gamma, EF-1-gamma, EF1G

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Elongation factor 1-gamma Antibody (A90247)

Western blot analysis of extracts of various cell lines, using Anti-Elongation factor 1-gamma Antibody (A90247) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-Elongation factor 1-gamma Antibody (A90247)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Elongation factor 1-gamma Antibody (A90247) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Elongation factor 1-gamma Antibody (A90247)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Elongation factor 1-gamma Antibody (A90247) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Elongation factor 1-gamma Antibody (A90247)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Elongation factor 1-gamma Antibody (A90247) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.