Anti-EPF Antibody [ARC1411] (A306116)

Name: Anti-EPF Antibody [ARC1411]
Brand: Antibodies.com
SKU: A306116
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1411] antibody to EPF for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-EPF Antibody [ARC1411]

Description

Rabbit monoclonal [ARC1411] antibody to EPF.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human HSPE1/HSP10/CPN10 (P61604).

Sequence

GSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1411

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

11 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

10 kDa chaperonin, 10 kDa heat shock protein, mitochondrial, Chaperonin 10, CPN10, Early-pregnancy factor, Heat shock protein family E member 1, Hsp10, HSPE1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-EPF Antibody [ARC1411] (A306116)

Western blot analysis of extracts of various cell lines, using Anti-EPF Antibody [ARC1411] (A306116) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-EPF Antibody [ARC1411] (A306116)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-EPF Antibody [ARC1411] (A306116) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-EPF Antibody [ARC1411] (A306116)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-EPF Antibody [ARC1411] (A306116) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-EPF Antibody [ARC1411] (A306116)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-EPF Antibody [ARC1411] (A306116) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.