The antibody detects endogenous levels of EPHA2 / 3 / 4 only when phosphorylated at tyrosine 588 / 596
Applications
WB, IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 588 / 596 (K-T-Y(p)-V-R) / (R-T-Y(p)-V-D) derived from Human EPHA2 / 3 / 4.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.
Publishing research using Anti-EPHA2 / 3 / 4 (Phospho-Tyr588 / 596) Antibody (A50527)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-EPHA2 / 3 / 4 (Phospho-Tyr588 / 596) Antibody (A50527)
