The antibody detects endogenous levels of EPHA3 / 4 / 5 only when phosphorylated at tyrosine 779 / 833.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 779 / 833 (E-A-Y(p)-T-T) / (A-A-Y(p)-T-T) derived from Human EPHA3 / 4 / 5.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody #11729 (left)or the same antibody preincubated with blocking peptide (right).
Publishing research using Anti-EPHA3 / 4 / 5 (Phospho-Tyr779 / 833) Antibody (A51417)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-EPHA3 / 4 / 5 (Phospho-Tyr779 / 833) Antibody (A51417)