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Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A93432)

Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.com Immunohistochemistry - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.com Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to Ephrin B1 + B2 + B3 (phospho Tyr324) for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody
Description
Rabbit polyclonal antibody to Ephrin B1 + B2 + B3 (phospho Tyr324).
Specificity
This antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324 (amino acids 290-339).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
38kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.comEnlarge Image

Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A93432)

Western blot analysis of lysates from K562 cells treated with serum 20% 15' using Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A93432)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A8281) - Antibodies.comEnlarge Image

Western Blot - Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody (A93432)

Western blot analysis of JK cells using Anti-Ephrin B1 + B2 + B3 (phospho Tyr324) Antibody.
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