Rabbit polyclonal antibody to EPS15 (Phospho-Tyr849)
Specificity
The antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
AF1P, Epidermal growth factor receptor substrate 15, Protein AF-1p, Protein Eps15
Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody #11800. The lane on the left is treated with antigen-specific peptide.
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