Mouse monoclonal [24D2] antibody to ErbB2/HER2 (APC).
Specificity
This antibody recognizes an extracellular epitope of human CD340, a type I transmembrane protein expressed by mesenchymal stem cells, B-lymphoblastic leukemia cells, subsets of C-ALL blasts, and various types of carcinomas.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH-3T3 / human CD340 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
24D2
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of MCF-7 cells stained with Anti-ErbB2/HER2 Antibody [24D2] (APC) (A121905), (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from MCF-7 cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (APC) (A86198), (concentration in sample 3 µg/ml, same as CD340 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of MCF-7 cell suspension.
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